Today at Berkeley Lab

Freeze-Frame Microscopy Captures Molecule’s ‘Lock-and-Load’ on DNA

Eva Nogales, faculty scientist in the Biosciences Area, led a team that captured freeze-frames of the changing shape of the huge macromolecular complex, transcription factor IID, as it locks onto DNA and loads the machinery for reading the genetic code. Scientists used cryoelectron microscopy (cryo-EM) to obtain snapshots of this structure as it engages with DNA, scans the sequence, and recruits and rearranges the appropriate proteins. More>